Effects of Phosphatidylinositol 3-Kinase Inhibitors, Wortmannin and LY294002, on Germinal Vesicle Breakdown (GVBD) in Porcine Oocytes.
نویسندگان
چکیده
منابع مشابه
Microtubules during germinal vesicle breakdown (GVBD) of Xenopus oocytes: effect of Ca2+ ionophore A-23187 and taxol.
During the first meiotic cell division of the Xenopus oocyte, a transient microtubule network appears at the basal part of the disintegrating nucleus. In order to know if this structure plays a role in the formation of the first meiotic spindle, we have studied the actions of Ca2+ ions and taxol during the whole maturation process. We now report that when the free cytosolic Ca2+ concentration i...
متن کاملProtein requirements for germinal vesicle breakdown in ovine oocytes.
The regulation of the cell cycle during the transition from prophase to metaphase I was studied by analysing protein changes and introducing protein blocks during the transition phase. The results show that the progression to metaphase in ovine oocytes is totally dependent on new protein synthesis. By delaying the addition of the inhibitor, cycloheximide, for progressively longer periods after ...
متن کاملHistone H1 kinase activity, germinal vesicle breakdown and M phase entry in mouse oocytes.
Meiotic reinitiation of the mouse oocyte is characterized by a slow entry into metaphase I, beginning with germinal vesicle breakdown and ending with spindle formation. It is accompanied by a cascade of protein kinases and phosphatases increasing protein phosphorylation. The activation of histone H1 kinase and that of the mitogen-activated protein kinase p42 have been compared during spontaneou...
متن کاملPossible involvement of phosphatidylinositol 3-kinase, but not protein kinase B or glycogen synthase kinase 3beta, in progesterone-induced oocyte maturation in the Japanese brown frog, Rana japonica.
It is known that amphibian oocytes undergo maturation through the formation and activation of maturation-promoting factor (MPF) in response to stimulation by the maturation-inducing hormone progesterone; however, the signal transduction pathway that links the hormonal stimulation on the oocyte surface to the activation of MPF in the oocyte cytoplasm remains a mystery. The aim of this study was ...
متن کاملWortmannin, a potent and selective inhibitor of phosphatidylinositol-3-kinase.
Phosphatidylinositol-3-kinase is an important enzyme for intracellular signaling. The microbial product wortmannin and some of its analogues have been shown to be potent inhibitors of phosphatidylinositol-3-kinase. The 50% inhibitory concentration for inhibition by wortmannin is 2 to 4 nM. Kinetic analysis demonstrates that wortmannin is a noncompetitive, irreversible inhibitor of phosphatidyli...
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ژورنال
عنوان ژورنال: Journal of Reproduction and Development
سال: 1998
ISSN: 0916-8818,1348-4400
DOI: 10.1262/jrd.44.281